Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV)
نویسندگان
چکیده
منابع مشابه
Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana
Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...
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Sugarcane mosaic caused by Sugarcane Mosaic Virus (SCMV) is one of the most important virus diseases of sugarcane. In the present study, changes in the transcription profile obtained by cDNA-AFLP analysis were investigated in two sugarcane varieties contrasting to SCMV resistance, when challenged with a severe virus strain. Healthy plants derived from meristem tip tissue culture were mechanical...
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zki (1993) noted that genetic engineering in sugarcane would be a useful tool for reversing single flaws, such Genetic transformation of sugarcane (Saccharum spp.) holds promas disease susceptibility, in commercial cultivars. Sugarise for increasing yields and disease resistance. However, the tissue cane may benefit from genetic transformation because its culture and transformation process may ...
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Sugarcane (Saccharum spp. hybrids) is a world-wide cash crop for sugar and biofuel in tropical and subtropical regions and suffers serious losses in cane yield and sugar content under salinity and drought stresses. Although real-time quantitative PCR has a numerous advantage in the expression quantification of stress-related genes for the elaboration of the corresponding molecular mechanism in ...
متن کاملHot Water Treatment of Cutting-cane Infected with Sugarcane Streak Mosaic Virus (scsmv)
The thermal inactivation point (TIP) of SCSMV was determined by heating the infected plant sap for 10 min at 50oC, 55oC, 60oC, and 65oC. The sap was inoculated mechanically on indicator plant Dactylactonium aegypticum. The virus was detected by a reverse transcription polymerase chain reaction (RT-PCR) using specific primer to amplify a part of the coat protein (CP) gene. The SCSMV was detected...
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ژورنال
عنوان ژورنال: BMC Research Notes
سال: 2019
ISSN: 1756-0500
DOI: 10.1186/s13104-019-4168-5